Selected for republication in the virtual journal for biomedical optics, vol. We have been working with this data, and starting to develop algorithms, implementations, and techniques for reducing this data. Wide area imaging of ultrasonic lamb wave fields by electronic speckle pattern interferometry grant a. The widefield imaging interferometry testbed1 arxiv. Douglas mast applied research laboratory, the pennsylvania state university p. Principles of qpi a conventional brightfield imaging measures. Kim university of south florida, department of physics, 4202 e. The wide field imaging interferometry testbed wiit is a wide field spectral imaging michelson interferometer developed at the nasagoddard space flight center. Nonflat sky over large fov rather technical, and only a problem for widefield imaging 4. Focal plane arrays for wide field imaging, spectroscopy, and. The speckle techniques can be classified into three broad categories. Electronic laser speckle interferometer for displacement. Quantitative phase microscopy of biological cell dynamics by.
Focal plane arrays for wide field imaging, spectroscopy, and interferometry we are developing large format up to 2,560 pixel mosaic arrays for farinfrared wavelengths to increase the utilization of valuable focal plane real estate made possible with modern telescopes. In recent years, with the advent of digital processing techniques, full field. We introduce a new shearing interferometry module for digital holographic microscopy, in which the offaxis angle, which defines the interference fringe frequency, is not coupled to the shearing distance, as is the case in most shearing interferometers. These techniques will be important for a wide range of future space based interferometry missions. Recent progress in widefield imaging interferometry. Nonflat sky over large fov rather technical, and only a problem for wide field imaging 4. Pdf quantitative microscopy and nanoscopy of sickle red. Interferometric phase measurements of wide field images of biological cells provide a quantitative tool for cell biology, as well as for medical diagnosis and monitoring. In wide field digital interferometry wfdi with offaxis geometry, the entire wavefront is captured in a single camera exposure. Jan 01, 2010 quantitative phase microscopy of articular chondrocyte dynamics by widefield digital interferometry shaked, natan t. Digital holography and its multidimensional imaging. Using the scanless interferometric system, we visualize chondrocyte swelling.
We present the first realization of a widefield su1,1 interferometer, where the use of a focusing element enables. The widefield imaging interferometry testbed wiit was designed to validate, experiment with, and refine the technique of wide field mosaic imaging for opticalir interferometers. Speckle photography 2 includes the methods where positional changes of the speckle are monitored. Nanoparticle classification in widefield interferometric. The wide field imaging interferometry testbed wiit was designed to validate, experiment with, and refine the technique of wide field mosaic imaging for opticalir interferometers.
The widefield imaging interferometry testbed wiit is a widefield spectral imaging michelson interferometer designed and developed at the nasagoddard space flight center. Principles and techniques of digital holographic microscopy. Astronomical interferometry, fourier transform spectroscopy, wide field imaging, hyperspectral image projectors 1. Digital holography dh 1016 is a technique in which a digital hologram that contains an object wavefront is recorded, and both 3d and quantitative phase images of an object are reconstructed using a computer. Pdf interferometric based measurement techniques have long played a significant. In recent years, with the advent of digital processing techniques, full field quantitative data can be obtained from interferometric data with relative ease and a high degree of accuracy. We extend compressed sensing techniques for interferometric imaging to a wfov and recover images in the spherical coordinate space in which they naturally live, eliminating any distorting projection. S o1,2,3,5, 1laser biomedical research center, massachusetts institute of technology, cambridge. Nearcommonpath interferometer for imaging fouriertransform spectroscopy in widefield microscopy dushan n. Digital holography is an emerging field of new paradigm in general imaging applications.
Widearea imaging of ultrasonic lamb wave fields by. Field stop filter interference filters all but the red. Nanoparticle classification in widefield interferometric microscopy by supervised learning from model oguzhan avci,1 celalettin yurdakul,1 and m. The detector array packages are compact, robust, light. Wide bandwidth filter 300nm white light in vsi 17 microscope diagram digitized intensity data beamsplitter detector array illuminator microscope objective translator mirau interferometer light source aperture stop field stop filter interference filters all but the red light from white light of halogen lamp. Topography measurement and digital refocusing requires both ampli tude and phase. Modeling the thermalmechanical coupling of samples, we design, build, and validate an iroptical hybrid iroh microscope that uses optical interferometry to measure the dimensional change in materials arising from spectral absorption. The use of multiple wells in the camera enables extremely rapid measurement of a full phase field at high resolution and the modulation frequency is not limited by the camera frame. The basic function of optical systems for multiplebeam interferometry is to allow observation of the interference pattern appearing in the wedge space formed by two surfaces. The data acquired with the wiit is double fourier data, including both spatial and spectral information within each data cube.
Quantitative microscopy and nanoscopy of sickle red blood cells performed by wide field digital interferometry article pdf available in journal of biomedical optics 163. Rapid widefield heterodyne interferometry with custom 2d. Demonstration of the widefield imaging interferometer. A twobeam interferometer functions by dividing originally coherent light into two beams of equal intensity, directing one beam onto the reference mirror and the other onto the specimen, and measuring the optical path difference the difference in optical distances between the resulting two reflected light waves. Thus, it enables the selection of shearing distance based on the spatial density of the sample, without losing spatial frequency content due. The balloon experimental twin telescope for infrared. Mathematical models for wide field doublefourier interferometric imaging have already been developed by lyon et al. The emerging astronomical technique known as wide field spatiospectral interferometry can provide hyperspectral images with spatial resolutions that are unattainable with a single monolithicaperture observatory. To extend the application space of the standard ldv to monitor multiple points widefield, the laser beam can be scanned over the surface. Principles and techniques of digital holographic microscopy myung k. The widefield imaging interferometry testbed wiit at nasas goddard space flight center and a computational model of the testbed were developed to demonstrate and learn the practical limitations of techniques for wide field spatialspectral double fourier interferometry.
A widefield pseudoheterodyne interference contrast microscope is described, which employs a complementary metaloxide semiconductor cmos phasesensitive camera. Wiit is operational and effectively demonstrates imaging and spectroscopy over fieldsofview larger than the narrow primary beam footprint of a conventional michelson interferometer. We experimentally implement labelfree phase microscopy using wide field digital interferometry wfdi techniques to retrieve quantitative volumetric data of articular chondrocyte dynamics. This study reports the ability to provide labelfree molecular information from infrared ir spectroscopy via the ubiquitous optical microscope. Wiit is now operational and is being used to demonstrate imaging and spectroscopy over fieldsofview larger than the typically narrow primary beam footprint of a. Wholecellanalysis of live cardiomyocytes using widefield.
Widefield imaging interferometry spatialspectral image. S o1,2,3,5, 1laser biomedical research center, massachusetts institute of technology, cambridge, massachusetts 029, usa 2department of biological engineering. Concluding remarks as optical profilers continue to improve they become more attractive metrology solutions for a number of. A wide field pseudoheterodyne interference contrast microscope is described, which employs a complementary metaloxide semiconductor cmos phasesensitive camera. Wide field digital interferometry wfdi is a quantitative holographic approach 9 that is capable of capturing the entire complex field distribution amplitude and phase associated with a sample in a single digital camera exposure, without the need for scanning or contrast. Interferometry is an important investigative technique in the fields of astronomy, fiber optics, engineering metrology, optical metrology, oceanography. Interferometry is a family of techniques in which waves, usually electromagnetic waves, are superimposed, causing the phenomenon of interference, which is used to extract information. Using the scanless interferometric system, we visualize chondrocyte swelling and bursting induced by hypoosmotic pressure. The wiit combines stellar interferometry with fourier. May 30, 2007 interferometric based measurement techniques have long played a significant role in enabling insight and understanding into the flow and heat transfer processes in a wide range of fluid flow situations. The wide field imaging interferometry testbed wiit was designed to develop techniques for wide field of view imag ing interferometry, using doublefourier methods.
Finan, 1, 2 farshid guilak, 1, 2 and adam wax 1 1 duke university, department of biomedical engineering, fitzpatrick institute for photonics, durham, north carolina 27708. Nearcommonpath interferometer for imaging fouriertransform spectroscopy in wide field microscopy dushan n. Rapid widefield heterodyne interferometry with custom 2d cmos camera j. Wiit operates at optical wavelengths and uses michelson beam combination to achieve both wide field imaging and highresolution. Quantitative phase microscopy of articular chondrocyte dynamics by. The widefield imaging interferometer testbed wiit at nasas goddard space flight center uses a dualmichelson interferometric technique. In offaxis configuration, with a slow camera and a laser diode, holographic interferometry is sensitive enough to enable wide field, laser doppler imaging of optical fluctuations in amplitude and phase, either with a slow or a fast camera.
This enables one to capture both the amplitude and the phase distributions of rapid 3d dynamic phenomena. The combination of an 8meter baseline with a doublefourier michelson interferometer allows. Two ways of understanding interferometry optics put a mask over an aperture. The wide field imaging interferometry testbed wiit is a wide field spectral imaging michelson interferometer designed and developed at the nasagoddard space flight center.
Shaked, continuous widefield characterization of drug release from skin substitute using offaxis interferometry, optics letters, vol. Digital speckle pattern heterodyne interferometry stanford university. Nearcommonpath interferometer for imaging fouriertransform. The theoretical groundwork for operation and data measurement is presented in full detail, including relevant coherence theory. Osa quantitative phase imaging by widefield interferometry.
Quantitative phase microscopy of articular chondrocyte dynamics by wide field digital interferometry natan t. Alldigital histopathology by infraredoptical hybrid. Here we offer motivation for wiit, present the testbed design, and describe algorithms that can be used to reduce the data from a spatial and spectral michelson. Quantitative phase microscopy of articular chondrocyte. The balloon experimental twin telescope for infrared interferometry bettii is a new balloonborne farinfrared interferometer, being designed to provide spatiallyresolved spectroscopy in the far infrared 3090. Primary beam maximum field of view set by the telescope aperture. The use of multiple wells in the camera enables extremely rapid measurement of a full phase field at high resolution and the modulation frequency is not limited by the camera.